0727. Normal saline versus ringer´s lactate in experimental sepsis

Methods Fourteen adult sheep (24-34 Kg) were anesthetized (midazolam, ketamine and morphine), mechanically ventilated and invasively monitored. A cecotomy was performed to collect autologous feces that were later reinjected into the peritoneal cavity through the abdominal wall to create abdominal sepsis. RL was administered during the surgical procedure. After baseline measurements, animals were randomly allocated to receive only NS or RL titrated to maintain pulmonary artery occlusion pressure at baseline level. Neither vasoactive agents nor antibiotics were used during the experiment. Animals were followed until death or for a maximum of 30 hours. Time-evolution for repeated measurement data was analyzed using a Generalized Estimating Equations approach in SPSS 19.0 (IBM) with a p < 0.05 considered as significant. Data are presented as median with inter-quartile range.